Monoclonal Antibody Production

As we study in Unit 4 Ch 17, about Monoclonal Antibody and how it's prepare only one type of antibody, derived from a single cloned B cell. The first step is to inject an animal with a antigen of specific epitope of interest. Then the B cell isolated from the spleen and then mixed with myeloma cell. Those two type of cells are fuse together to form hybridomas cells. Then the hybridomas cells put on petri dish culture under condition which permit only to growth the hybridomas cells. You will produce a single type of antibody against a single epitope, then you will separate the single hybridomas cells into individual wells of a microtiter plate to test for ability to produce preferred the antibody. Then produce monoclonal antibody and finally are isolate and purified.


http://www.1lecture.com/Immunology/Monoclonal%20Antibodies/index.html